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FastQ Screen allows you to screen a library of sequences in FastQ format against a set of sequence databases so you can see if the composition of the library matches with what you expect.
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FROM ubuntu:18.04
# Set working directory
WORKDIR /opt
# Updating ubuntu, installing other necessary software and remove tmp files
RUN apt-get update --yes \
&& apt-get install wget unzip python perl build-essential zlib1g-dev gzip libncurses5-dev libbz2-dev liblzma-dev libgd-dev --yes \
&& apt-get clean \
&& rm -rf /var/lib/apt/lists/* /tmp/* /var/tmp/*
# Install bowtie and remove tmp files
RUN wget https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.2.2/bowtie-1.2.2-linux-x86_64.zip \
&& unzip bowtie-1.2.2-linux-x86_64.zip \
&& rm bowtie-1.2.2-linux-x86_64.zip \
&& cd bowtie-1.2.2-linux-x86_64
ENV PATH="/opt/bowtie-1.2.2-linux-x86_64:${PATH}"
# Install bowtie2 and remove tmp files
RUN wget https://sourceforge.net/projects/bowtie-bio/files/bowtie2/2.3.5.1/bowtie2-2.3.5.1-linux-x86_64.zip \
&& unzip bowtie2-2.3.5.1-linux-x86_64.zip \
&& rm bowtie2-2.3.5.1-linux-x86_64.zip \
&& cd bowtie2-2.3.5.1-linux-x86_64
ENV PATH="/opt/bowtie2-2.3.5.1-linux-x86_64:${PATH}"
# Install BWA and remove tmp files
RUN wget https://sourceforge.net/projects/bio-bwa/files/bwa-0.7.17.tar.bz2 \
&& tar -xvf bwa-0.7.17.tar.bz2 \
&& rm bwa-0.7.17.tar.bz2 \
&& cd bwa-0.7.17 \
&& make
ENV PATH="/opt/bwa-0.7.17:${PATH}"
# Install SAMtools and remove tmp files
RUN wget https://github.com/samtools/samtools/releases/download/1.9/samtools-1.9.tar.bz2 \
&& tar jxf samtools-1.9.tar.bz2 \
&& rm samtools-1.9.tar.bz2 \
&& cd samtools-1.9 \
&& make \
&& make install
# Install GD::Graph and remove tmp files
# Install necessary modules for GD::Graph
RUN yes | perl -MCPAN -e "install Test::Fork; install Capture::Tiny; install Test::Exception"
# Install ExtUtils-PkgConfig
RUN wget https://cpan.metacpan.org/authors/id/X/XA/XAOC/ExtUtils-PkgConfig-1.15.tar.gz \
&& tar xvzf ExtUtils-PkgConfig-1.15.tar.gz \
&& rm ExtUtils-PkgConfig-1.15.tar.gz \
&& cd ExtUtils-PkgConfig-1.15 \
&& perl Makefile.PL \
&& make \
&& make install
# Install GD-2.70
RUN wget https://cpan.metacpan.org/authors/id/R/RU/RURBAN/GD-2.70.tar.gz \
&& tar xvzf GD-2.70.tar.gz \
&& rm GD-2.70.tar.gz \
&& cd GD-2.70 \
&& yes | perl Makefile.PL \
&& make \
&& make install
# Install GDTextUtil
RUN wget https://cpan.metacpan.org/authors/id/M/MV/MVERB/GDTextUtil-0.86.tar.gz \
&& tar xvzf GDTextUtil-0.86.tar.gz \
&& rm GDTextUtil-0.86.tar.gz \
&& cd GDTextUtil-0.86 \
&& perl Makefile.PL \
&& make \
&& make install
# Install GD::Graph
RUN wget https://cpan.metacpan.org/authors/id/R/RU/RUZ/GDGraph-1.54.tar.gz \
&& tar xvzf GDGraph-1.54.tar.gz \
&& rm GDGraph-1.54.tar.gz \
&& cd GDGraph-1.54 \
&& yes | perl Makefile.PL \
&& make \
&& make install
# Install Bismark and remove tmp files
RUN wget https://www.bioinformatics.babraham.ac.uk/projects/bismark/bismark_v0.22.1.tar.gz \
&& tar xvzf bismark_v0.22.1.tar.gz \
&& rm bismark_v0.22.1.tar.gz \
&& cd bismark_v0.22.1
ENV PATH="/opt/bismark_v0.22.1:${PATH}"
# Install FastQ Screen and remove tmp files
RUN wget https://www.bioinformatics.babraham.ac.uk/projects/fastq_screen/fastq_screen_v0.14.0.tar.gz \
&& tar xvzf fastq_screen_v0.14.0.tar.gz \
&& rm fastq_screen_v0.14.0.tar.gz \
&& cd fastq_screen_v0.14.0
ENV PATH="/opt/fastq_screen_v0.14.0:${PATH}"
# Set command to bash
CMD ["/bin/bash"]